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Image Search Results
Journal: Biophysical Journal
Article Title: Birefringence Changes of Dendrites in Mouse Hippocampal Slices Revealed with Polarizing Microscopy
doi: 10.1016/j.bpj.2020.03.016
Figure Lengend Snippet: A retardance map (A) and a combination map (B) of retardance (brightness) and slow axis orientation (color) of mouse hippocampus slice immersed in ACSF with 54% (w/v) of iodixanol. An bjective lens, Olympus XLUMPLANFL 20×, NA 0.95, was used. Scale bars, 100 μm. In (A), white arrowheads indicate bright streaks running parallel to the stratum radiatum, and black arrowheads indicate dark streaks running perpendicular to the stratum radiatum. Scale bars, 100 μm. The color wheel in (B) on the top right shows the reference for the local slow axis orientation of birefringence. (C) Transmitted light and (D) LC-PolScope image of primary cultured hippocampal neurons show a cell body (right top) and neurites. An objective lens, PlanApo VC 60×, NA 1.4 (Nikon), was used. Scale bars, 10 μm. In (D), the color (hue) indicates the slow axis orientation, and the brightness indicates the retardance value. An area enclosed by a white square in (D) is magnified as an inset at the left-bottom corner. The color wheel on the top right shows the reference for the local slow axis orientation of birefringence. To see this figure in color, go online.
Article Snippet: For imaging birefringence of single neurons in hippocampus slices, we prepared ACSF with 54% of iodixanol by mixing 10× concentrated ACSF with 60% of
Techniques: Cell Culture